JAK1 Kinase Assay Service

Activity-based JAK1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
JAK1

Enzyme
Human JAK1

HGNC
JAK1

Synonym
JAK1A, JAK1B, JTK3

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.1
AG1478 14,200
D-64406 11,800
Scientific Information
P23458 (http://www.uniprot.org/uniprot/P23458)

Screening Location
Malvern, USA

Reference compound IC50 for JAK1
Reference compound IC50 for JAK1

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