Activity-based JAK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JAK2
Enzyme
Human JAK2
HGNC
JAK2
Synonym
JAK2
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.1 |
| AG1478 | >20,000 |
| D-64406 | 15,000 |
Scientific Information
O60674 (http://www.uniprot.org/uniprot/O60674)
Screening Location
Malvern, USA
Reference compound IC50 for JAK2
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