JAK2 (V617F) Kinase Assay Service

Activity-based JAK2 (V617F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
JAK2 (V617F)

Enzyme
Human JAK2 (V617F)

HGNC
JAK2 (V617F)

Synonym
JAK2

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 1.4
PP2 >100,000
AG1478 >100,000
EGFR/ErbB Inhibitor >100,000
D-64406 16,000
Scientific Information
O60674 (http://www.uniprot.org/uniprot/O60674)

Screening Location
Malvern, USA

Reference compound IC50 for JAK2 (V617F)
Reference compound IC50 for JAK2 (V617F)

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