Activity-based JNK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
JNK1
Enzyme
Human JNK1, wt
HGNC
MAPK8
Manning ID
JNK1
Synonym
JNK, JNK1A2, JNK21B1/2, PRKM8, SAPK1, MAPK8, JNK1-alpha1, JNK1
UniProt
P45983 (MK08_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ATF2, ATP
Reaction
ATF2 + 33P-ATP = 33P-ATF2 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 613 |
| Sorafenib | 10000 |
| Sunitinb | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | JNK1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – JNK1 |
| Product Number | 0458-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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