Activity-based JNK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
JNK3
Enzyme
Human JNK3, wt
HGNC
MAPK10
Manning ID
JNK3
Synonym
JNK3A, PRKM10, p493F12, FLJ12099, p54bSAPK, MAPK10, JNK3
UniProt
P53779 (MK10_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CMGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ATF2, ATP
Reaction
ATF2 + 33P-ATP = 33P-ATF2 + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 680 |
| Sorafenib | 10000 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | JNK3 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – JNK3 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0900-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF