Activity-based JNK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
JNK3
Enzyme
Human JNK3
HGNC
MAPK10
Synonym
JNK3A, PRKM10, p493F12
Substrates
ATF2, 5 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 710 |
| Ro 31-8220 | 6,800 |
| GW5074 | 3,900 |
| JNKi VIII | 7.2 |
| SP600125 | 98 |
Scientific Information
P53779 (http://www.uniprot.org/uniprot/P53779)
Screening Location
Malvern, USA
Reference compound IC50 for JNK3
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