Activity-based KAT8 Histone Acetyltransferase Assay for compound screening and profiling via radiometric HAT assay.
Target Overview
CAT#
KAT8
Enzyme
Human KAT8
Synonyms
Histone acetyltransferase KAT8, MOF, MYST1
Substrates
Histone H4, 2.5 μM
Acetyl CoA, 3 μM
Assay Properties
Reaction
[Acetyl-³H]-Acetyl Coenzyme A + histone L-lysine = Coenzyme A + [Acety-³H]-L-lysine
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| WM-8014 | >10,000 |
| WM-3835 | >10,000 |
| PF-9363 | >10,000 |
| WM-1119 | >10,000 |
| Anacardic Acid | 13,000 |
| Garcinol | 12,000 |
| Curcumin | >100,000 |
| C646 | 4,000 |
Scientific Information
Q9H7Z6 (https://www.uniprot.org/uniprotkb/Q9H7Z6)
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Acetyltransferase Assay (HAT) Services for Drug Discovery
Reference Compound IC50 for KAT8
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