KDM4C/JMJD2C Demethylase Assay for compound screening and profiling via HTRF-based quantification of enzyme activity.
Target Overview
CAT#
KDM4C
Enzyme
Human KDM4C
Synonym
Lysine-specific demethylase 4C, Jumonji Domain-Containing Protein 2C, JHDM3C
Scientific Information
Q9H3R0 (https://www.uniprot.org/uniprot/Q9H3R0)
Assay Properties
Substrate
Biotin-H3(1-44)-K36Me3, α-KG
Reaction
H3(1-44)-K36Me3 + α-KG + O₂ = H3(1-44)-K36Me2 + succinate + CO₂ + formaldehyde
Detection
HTRF
Reference Compound IC50s
| Compound | IC50 (nM) |
|---|---|
| 2,4 PDCA | 327 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Histone Demethylase Assays for Drug Discovery
Reference compound IC50 for KDM4C/JMJD2C
