KDM6B Demethylase Assay for compound screening and profiling via AlphaLISA-based quantification of enzyme activity.
Target Overview
CAT#
KDM6B
Enzyme
Human KDM6B
Synonym
Lysine-specific demethylase 6B, JMJD3
Substrates
B- H3(21-44)-K27Me3, α-KG
Assay Properties
Reaction
H3(21-44)-K27Me3 + α-KG + O₂ = H3(21-44)-K27Me2/1 + succinate + CO₂ + formaldehyde
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| GSK-J1 | 12 |
Scientific Information
O15054 (http://www.uniprot.org/uniprot/O15054)
Screening Location
Malvern, USA
Reference compound inhibition of KDM6B
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