KHS/MAP4K5 Kinase Assay Service

Activity-based KHS/MAP4K5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
KHS/MAP4K5

Enzyme
Human KHS/MAP4K5

HGNC
MAP4K5

Synonym
KHS1, GCKR, KHS, MAPKKKK5

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 0.6
Ro 31-8220 260
GW 5074 7,600
H-89 38,000
Rottlerin 68,000
Scientific Information
Q9Y4K4 (http://www.uniprot.org/uniprot/Q9Y4K4)

Screening Location
Malvern, USA

Reference compound IC50 for KHS/MAP4K5
Reference compound IC50 for KHS/MAP4K5

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