Activity-based KIF10 (CENP-E) Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF10
Enzyme
Human KIF10 (1-395aa)
Family
Kinesin-7
Synonym
Centromere-associated protein E, Kinesin-7
Scientific Information
Q02224 (http://www.uniprot.org/uniprot/Q02224)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| GSK-923295 | 65.2 |
| PF-2771 | 6.6 |
| Sovilnesib (AMG 650) | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF10
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