Activity-based KIF11 (Eg5) Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF11
Enzyme
Human KIF11 (13-437aa)
Family
Kinesin-5
Synonym
EG5, KNSL1, TRIP5
Scientific Information
P52732 (http://www.uniprot.org/uniprot/P52732)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| EMD534085 | 3.1 |
| ARQ-621 | 1.7 |
| Ispinesib (SB-715992) | 0.4 |
| BRD9876 | 21 |
| Dimethylenastron | 65 |
| Sovilnesib (AMG 650) | >100,000 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF11
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