Activity-based KIF18A Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF18A
Enzyme
Human KIF18A (1-374aa)
Family
Kinesin-8
Synonym
Kinesin-like protein KIF18A, Marrow stromal KIF18A (MS-KIF18A)
Scientific Information
Q8NI77 (http://www.uniprot.org/uniprot/Q8NI77)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| BTB-1 | 535 |
| KIF18A-IN-1 | 260 |
| Sovilnesib (AMG 650) | 83 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF18A
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