Activity-based KIF23 (MKLP1) Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF23
Enzyme
Human KIF23 (17-502aa)
Family
Kinesin-6
Synonym
MKLP1, KNSL5
Scientific Information
Q02241 (http://www.uniprot.org/uniprot/Q02241)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Ispinesib (SB-715992) | >100 |
| GSK-923295 | >100 |
| Sovilnesib | >100 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF23
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