Activity-based KIF4A Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIF4A
Enzyme
Human KIF4A (3-418aa)
Family
Kinesin-4
Synonym
Chromokinesin-A, Chromosome-associated kinesin KIF4A
Scientific Information
O95239 (http://www.uniprot.org/uniprot/O95239)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Paprotrain | 14 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIF4A
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