Activity-based KIFC3 Kinesin ATPase assay for compound screening and profiling via ADP-Glo™.
Target Overview
CAT#
KIFC3
Enzyme
Human KIFC3 (384-825aa)
Family
Kinesin-14
Synonym
Kinesin-like protein KIFC3
Scientific Information
Q9BVG8 (http://www.uniprot.org/uniprot/Q9BVG8)
Assay Properties
Substrates
Tubulin Microtubules, 0.1 mg/ml
ATP, 10 µM
ATP, 10 µM
Reaction
ATP = ADP + Pi
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (µM) |
|---|---|
| Ispinesib (SB-715992) | 30.4 |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website Kinesin ATPase Assay Services
Reference compound inhibition of KIFC3
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