Activity-based KIT D816H kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
KIT D816H
Enzyme
Human KIT D816H, mutant
HGNC
KIT (D816E)
Manning ID
KIT
Synonym
KIT, C-Kit, PBT, CD117, SCFR, c-kit, KIT D816H, c-KIT (D816E)
UniProt
P10721 (KIT_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
PolyEY, ATP
Reaction
PolyEY + 33P-ATP = 33P-PolyEY + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 22 |
| Sorafenib | 3400 |
| Sunitinib | 940 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | KIT D816H |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | IC50 KIT Profiler |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – KIT D816H (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1041-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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