KIT (V559D,V654A) NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
KIT (V559D,V654A)
KIT (V559D/V654A)
C-Kit, PBT, CD117, SCFR
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-KIT(V559D,V654A) Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-4 and then treated with reference compound Dasatinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism 4 program based on a sigmoidal dose response equation.
Binding of compound to target kinase
| Compounds | IC50 (M) |
|---|---|
| Dasatinib | 3.249e-008 |
Malvern, USA
