KRAS-related Assays: K-Ras::SOS1 Protein-Protein Interaction Assay Service for compound screening and profiling using the HTRF assay format to measure the binding of K-Ras and SOS1
Target Overview
Target
Human SOS1, human K-Ras
HGNC
SOS1, KRAS
Protein
K-Ras (aa 2-169), biotinylated, untagged. Expressed in E. coli.
SOS1 (aa 564-1069), His/strep tagged. Expressed in E. coli.
Synonyms
SOS1: Son of sevenless homolog 1, GF1, GGF1, GINGF, HGF, NS4
K-Ras: C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2, Kras
Assay Properties
Scientific information
Screening location
Malvern, PA, US
Reaction
Binding of K-Ras-GDP to SOS1
Readout
HTRF
Reference compound EC50s
| Compound | EC50 (nM) |
|---|---|
| BAY-293 | 20 |
Reference compound BAY-293 inhibits the protein-protein interaction of K-Ras-GDP and SOS1
PDF