Activity-based KSR1 (L639F) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
KSR1 (L639F)
Enzyme
Human KSR1 (L639F)
HGNC
KSR1 (L639F)
Synonym
Kinase suppressor of Ras 1
Substrates
Peptide substrate, [KRREILSRRPSYR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 10,000 |
| Ro 31-8220 | 25,000 |
| GW5074 | 20,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q8IVT5 (http://www.uniprot.org/uniprot/Q8IVT5)
Screening Location
Malvern, USA
Reference compound IC50 for KSR1 (L639F)
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