KSR2 (R676S) Kinase Assay Service

Activity-based KSR2 (R676S) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
KSR2 (R676S)

Enzyme
Human KSR2 (R676S)

HGNC
KSR2 (R676S)

Synonym
Kinase suppressor of Ras 2

Substrates
Peptide substrate, [KRREILSRRPSYR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 6,100
Ro 31-8220 21,400
GW5074 16,600
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q6VAB6 (http://www.uniprot.org/uniprot/Q6VAB6)

Screening Location
Malvern, USA

Reference compound IC50 for KSR2 (R676S)
Reference compound IC50 for KSR2 (R676S)

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