LCK Kinase Assay Service

Activity-based LCK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
LCK

Enzyme
Human LCK

HGNC
LCK

Synonym
LCK

Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 6.6
PP2 170
AG1478 2,900
D-64406 4,300
Scientific Information
P06239 (http://www.uniprot.org/uniprot/P06239)

Screening Location
Malvern, USA

Reference compound IC50 for LCK
Reference compound IC50 for LCK

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