Activity-based LIMK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LIMK2
Enzyme
Human LIMK2
HGNC
LIMK2
Synonym
LIMK2
Substrates
Cofilin 2, 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 200 |
| Ro 31-8220 | 11,000 |
| GW5074 | 66,000 |
| H-89 | 14,000 |
| Rottlerin | 16,500 |
Scientific Information
P53671 (http://www.uniprot.org/uniprot/P53671)
Screening Location
Malvern, USA
Reference compound IC50 for LIMK2
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