LKB1 Kinase Assay Service

Activity-based LKB1 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
LKB1

Enzyme
Human LKB1 complex with STRADα/ MO25α

HGNC
STK11

Synonym
LKB1

Substrates
Peptide substrate, [LSNLYHQGKFLQTFCGSPLYRRR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 37.8
Ro 31-8220 4,300
GW5074 9,000
H-89 12,100
Scientific Information
Q15831 (http://www.uniprot.org/uniprot/Q15831)

Screening Location
Malvern, USA

Reference compound IC50 for LKB1
Reference compound IC50 for LKB1

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