Activity-based LOK/STK10 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LOK/STK10
Enzyme
Human LOK/STK10
HGNC
STK10
Synonym
LOK, PRO2729
Substrates
Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.5 |
| Ro 31-8220 | 45 |
| GW5074 | 1,900 |
| H-89 | 9,300 |
| Rottlerin | >100,000 |
Scientific Information
O94804 (http://www.uniprot.org/uniprot/O94804)
Screening Location
Malvern, USA
Reference compound IC50 for LOK/STK10
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