Activity-based LRRK2 I2020T kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
LRRK2 I2020T
Enzyme
Human LRRK2 I2020T, mutant
HGNC
LRRK2 (I2020T)
Manning ID
LRRK2
Synonym
PARK8, ROCO2, FLJ45829, DKFZp434H2111, LRRK2, LRRK2 I2020T, LRRK2 (I2020T)
UniProt
Q5S007 (LRRK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 7 |
| Sorafenib | 4000 |
| Sunitinib | 150 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | LRRK2 I2020T |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
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