LRRK2 (I2020T) Kinase Assay Service

Activity-based LRRK2 (I2020T) kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
LRRK2 (I2020T)

Enzyme
Human LRRK2 (I2020T)

HGNC
LRRK2 (I2020T)

Synonym
PARK8, ROCO2, FLJ45829, DKFZp434H2111

Substrates
Peptide substrate, [RLGRDKYKTLRQIRQ], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 3.0
Ro 31-8220 380
GW5074 39
H-89 >100,000
Rottlerin >100,000
Scientific Information
Q5S007 (http://www.uniprot.org/uniprot/Q5S007)

Screening Location
Malvern, USA

Reference compound IC50 for LRRK2 (I2020T)
Reference compound IC50 for LRRK2 (I2020T)

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