Activity-based LRRK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
LRRK2
Enzyme
Human LRRK2, wt
HGNC
LRRK2
Manning ID
LRRK2
Synonym
PARK8, ROCO2, FLJ45829, DKFZp434H2111, ROCO2, LRRK2, LRRK2 wt
UniProt
Q5S007 (LRRK2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-GSK3(14-27), ATP
Reaction
RBER-GSK3(14-27) + 33P-ATP = 33P-RBER-GSK3(14-27) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 5 |
| Sorafenib | 8000 |
| Sunitinb | 40 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | LRRK2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF