Activity-based LYNB kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
LYN B
Enzyme
Human LYN B
HGNC
LYN (d23-43)
Synonym
JTK8
Substrates
Peptide substrate, poly[Glu:Tyr] (4:1), 0.2 mg/ml
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 4.5 |
| PP2 | 520 |
| AG1478 | 1,900 |
| D-64406 | 3,300 |
Scientific Information
P07948-2 (http://www.uniprot.org/uniprot/P07948-2)
Screening Location
Malvern, USA
Further information
More information can be found on our website Kinase Assays.
Reference compound IC50 for LYNB
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