M2 muscarinic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
M2 Binding
GPCR Target
Human M2
Source
Membrane preparation from HEK293 cells stably transfected with human M2 receptor.
Scientific Information
ACM2 (https://www.uniprot.org/uniprotkb/P08172/entry)
Assay Properties
Protocol
Radioligand filter binding to M2 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| AF-DX384 (20.5) | AF-DX384 (5) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for M2
PDF