M3 muscarinic Biochemical Binding Assay for compound screening and profiling via competitive radioligand binding.
Target Overview
CAT#
M3 Binding
GPCR Target
Human M3
Source
Membrane preparation from HEK293 cells stably transfected with human M3 receptor.
Scientific Information
ACM3 (https://www.uniprot.org/uniprotkb/P20309/entry)
Assay Properties
Protocol
Radioligand filter binding to M3 membrane preparation ± cold competing ligand; radiometric measurement on MicroBeta scintillation counter
Readout
CPM representing 3H-disintegration detected using MicroScint-20
Reference Compound IC50
| Compound IC50 (nM) | Radioligand (nM) |
|---|---|
| 4-DAMP (13.7) | NMS (3) |
Screening Location
Malvern, PA, USA
Further Information
More information can be found on our website In Vitro Safety Pharmacology Profiling
Reference compound binding data for M3
PDF