MAK Kinase Assay Service

Activity-based MAK kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MAK

Enzyme
Human MAK

HGNC
MAK

Synonym
MAK

Substrates
MBP (Myelin Basic Protein), 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine 30
Ro 31-8220 0.4
GW5074 4,300
H-89 >100,000
Rottlerin >100,000
Scientific Information
P20794 (http://www.uniprot.org/uniprot/P20794)

Screening Location
Malvern, USA

Reference compound IC50 for MAK
Reference compound IC50 for MAK

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