Activity-based MAP3K10 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAP3K10
Enzyme
Human MAP3K10, wt
HGNC
MAP3K10
Manning ID
MLK2
Synonym
MST, MEKK10, MLK2, MEKK10, MAP3K10, MLK2/MAP3K10
UniProt
Q02779 (M3K10_HUMAN)
Kinase Type
Protein kinase
Kinase Group
TKL
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Histon H2B, ATP
Reaction
Histon H2B + 33P-ATP = 33P-Histon H2B + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 12 |
| Sorafenib | 10000 |
| Sunitinib | 40000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAP3K10 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF