Activity-based MAP3K1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAP3K1
Enzyme
Human MAP3K1, wt
HGNC
MAP3K1
Manning ID
MAP3K1
Synonym
MAPKKK1, MEKK, MEKK1, MAP3K1, MEKK1
UniProt
Q13233 (M3K1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
bio-4xCHKT, ATP
Reaction
bio-4xCHKT + 33P-ATP = 33P-bio-4xCHKT + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1200 |
| Sorafenib | 10000 |
| Sunitinib | 100000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAP3K1 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MAP3K1 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1103-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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