Activity-based MAP4K1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAP4K1
Enzyme
Human MAP4K1, wt
HGNC
MAP4K1
Manning ID
HPK1
Synonym
HPK1, MAP4K1, HPK1/MAP4K1
UniProt
Q92918 (M4K1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Casein, ATP
Reaction
Casein + 33P-ATP = 33P-Casein + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 0.7 |
| Sorafenib | 10000 |
| Sunitinb | 19 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAP4K1 |
| 345 wt Profiler | No |
| 345 IC50 wt Profiler | No |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MAP4K1 |
| Product Number | 1941-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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