Activity-based MAP4K2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAP4K2
Enzyme
Human MAP4K2, wt
HGNC
MAP4K2
Manning ID
GCK
Synonym
BL44, GCK, RAB8IP, MAP4K2, GCK/MAP4K2
UniProt
Q12851 (M4K2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
3xChocktide-PKC(19-31), ATP
Reaction
3xChocktide-PKC(19-31) + 33P-ATP = 33P-3xChocktide-PKC(19-31) + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 12 |
| Sorafenib | 8400 |
| Sunitinb | 1500 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAP4K2 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF