Activity-based MAPKAPK5 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MAPKAPK5
Enzyme
Human MAPKAPK5, wt
HGNC
MAPKAPK5
Manning ID
MAPKAPK5
Synonym
PRAK, MAPKAPK5, MAPKAPK5/PRAK
UniProt
Q8IW41 (MAPK5_HUMAN)
Kinase Type
Protein kinase
Kinase Group
CAMK
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-CHKtide, ATP
Reaction
RBER-CHKtide + 33P-ATP = 33P-RBER-CHKtide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 193 |
| Sorafenib | 10000 |
| Sunitinb | 23000 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MAPKAPK5 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MAPKAPK5 |
| Product Number | 0712-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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