Activity-based MARK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MARK3
Enzyme
Human MARK3
HGNC
MARK3
Synonym
CTAK1, KP78, PAR1A
Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | <0.5 |
| Ro 31-8220 | 2,500 |
| GW5074 | 6,300 |
| H-89 | 9,300 |
Scientific Information
P27448 (http://www.uniprot.org/uniprot/P27448)
Screening Location
Malvern, USA
Reference compound IC50 for MARK3
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