MARK4 Kinase Assay Service

Activity-based MARK4 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview

CAT#
MARK4

Enzyme
Human MARK4

HGNC
MARK4

Synonym
KIAA1860, MARKL1

Substrates
Peptide substrate, [KKKVSRSGLYRSPSMPENLNRPR], 20 µM
ATP 10 µM

Assay Properties

Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP

Readout
Enzymatic activity

Reference Compound IC50s
Compounds IC50 (nM)
Staurosporine <0.5
Ro 31-8220 3,300
GW5074 5,000
H-89 15,000
Scientific Information
Q96L34 (http://www.uniprot.org/uniprot/Q96L34)

Screening Location
Malvern, USA

Reference compound IC50 for MARK4
Reference compound IC50 for MARK4

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