Activity-based MAST3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MAST3
Enzyme
Human MAST3
HGNC
MAST3
Synonym
Microtubule-associated serine/threonine-protein kinase 3, KIAA0561
Substrates
ARPP19, 5 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1,900 |
| Ro 31-8220 | >100,000 |
| GW 5074 | 24,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
O60307 (http://www.uniprot.org/uniprot/O60307)
Screening Location
Malvern, USA
Reference compound IC50 for MAST3
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