Activity-based MASTL kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MASTL
Enzyme
Human MASTL, wt
HGNC
MASTL
Manning ID
MASTL
Synonym
FLJ14813, RP11-85G18.2, THC2, MASTL, GLW, GREATWALL, GWL, hGWL, MAST-L
UniProt
Q96GX5 (GWL_HUMAN)
Kinase Type
Protein kinase
Kinase Group
AGC
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-NTRK3tide, ATP
Reaction
RBER-NTRK3tide + 33P-ATP = 33P-RBER-NTRK3tide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 1000 |
| Sorafenib | 10000 |
| Sunitinb | 9200 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MASTL |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MASTL |
| Product Number |
1593-0000-2 |
| Web Shop | Reaction Biology Online Shop |
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