Activity-based MEK1 F53L kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MEK1 F53L
Enzyme
Human MEK1 F53L, mutant
HGNC
MAP2K1 (F53L)
Manning ID
MAP2K1
Synonym
MAP2K1, MAPKK1, MAPKK 1, MKK1, PRKMK1, MEK1 F53L
UniProt
Q02750 (MP2K1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ERK2-KR, ATP
Reaction
ERK2-KR + 33P-ATP = 33P-ERK2-KR + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 135 |
| Sorafenib | 10000 |
| Sunitinib | 840 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MEK1 F53L |
| 355 wt Profiler | No |
| 355 IC50 wt Profiler | No |
| Mutant Profiler Addon | Yes |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MEK1 F53L (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 1538-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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