MEK1 Kinase Activity Assay Service

Activity-based MEK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology

Target Overview
RBE Target ID

MEK1

Enzyme

Human MEK1, wt

HGNC

MAP2K1

Manning ID

MAP2K1

Synonym

MAPKK1, MKK1, PRKMK1, MAP2K1, MEK1 wt, MEK1

UniProt

Q02750 (MP2K1_HUMAN)

Kinase Type

Protein kinase

Kinase Group

STE

Assay Properties
Assay Format

33PanQinaseTM

Substrates

ERK2-KR, ATP

Reaction

ERK2-KR + 33P-ATP = 33P-ERK2-KR + ADP

Readout

Enzymatic activity

Technical Reference Compound IC50 value(s)
Compounds IC50 (nM)
Staurosporine 270
Sorafenib 10000
Sunitinib 4200
Screening Location

Freiburg, Germany

Further Information

More information can be found on our website Kinase Assays

Service and Product Availability
Screening Opportunities
FreeChoice MEK1
355 wt Profiler Yes
355 IC50 wt Profiler Yes
Mutant Profiler Addon No
Lipid Kinase Profiler Addon No
IC50 Profiler No
Recombinant Protein Availability
Active recombinant protein Yes; ProQinaseTM – MEK1
(Activity verified by 33PanQinaseTM-Assay)
Product Number 0550-0000-3
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