Activity-based MEK1 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MEK1
Enzyme
Human MEK1, wt
HGNC
MAP2K1
Manning ID
MAP2K1
Synonym
MAPKK1, MKK1, PRKMK1, MAP2K1, MEK1 wt, MEK1
UniProt
Q02750 (MP2K1_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
ERK2-KR, ATP
Reaction
ERK2-KR + 33P-ATP = 33P-ERK2-KR + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 270 |
| Sorafenib | 10000 |
| Sunitinb | 4200 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MEK1 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MEK1 |
| Product Number | 0550-0000-3 |
| Web Shop | Reaction Biology Online Shop |
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