Activity-based MEK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MEK2
Enzyme
Human MEK2, wt
HGNC
MAP2K2
Manning ID
MAP2K2
Synonym
MAPKK2, MKK2, PRKMK2, MEK2, PRKMK2, MAP2K2
UniProt
P36507 (MP2K2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
RBER-IRStide, ATP
Reaction
RBER-IRStide + 33P-ATP = 33P-RBER-IRStide + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 37 |
| Sorafenib | 10000 |
| Sunitinib | 5600 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MEK2 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | No |
| Product Number | N/A |
| Web Shop | Reaction Biology Online Shop |
PDF