Activity-based MEK2 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEK2
Enzyme
Human MEK2
HGNC
MAP2K2
Synonym
MAPKK2, MKK2, PRKMK2
Substrates
ERK2 (K52R), 1 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 9.6 |
| Ro 31-8220 | 8,500 |
| GW 5074 | 3,900 |
Scientific Information
P36507 (http://www.uniprot.org/uniprot/P36507)
Screening Location
Malvern, USA
Reference compound IC50 for MEK2
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