Activity-based MEK3 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEK3
Enzyme
Human MEK3
HGNC
MAP2K3
Synonym
MEK3
Substrates
p38a (K53A), 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 6.0 |
| Ro 31-8220 | 37,000 |
| GW5074 | >50,000 |
| H-89 | >50,000 |
| Rottlerin | >50,000 |
Scientific Information
P46734 (http://www.uniprot.org/uniprot/P46734)
Screening Location
Malvern, USA
Reference compound IC50 for MEK3
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