Activity-based MEK5 kinase assay for compound screening and profiling via radiometric HotSpotTM kinase assay.
Target Overview
CAT#
MEK5
Enzyme
Human MEK5
HGNC
MAP2K5
Synonym
MAPKK5, MKK5, PRKMK5
Substrates
ERK5 (K84R), 3 µM
ATP 10 µM
ATP 10 µM
Assay Properties
Reaction
Substrate + [γ-33P]-ATP = 33P-Substrate + ADP
Readout
Enzymatic activity
Reference Compound IC50s
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 52 |
| Ro 31-8220 | 1,100 |
| GW5074 | 15,000 |
| H-89 | >100,000 |
| Rottlerin | >100,000 |
Scientific Information
Q13163 (http://www.uniprot.org/uniprot/Q13163)
Screening Location
Malvern, USA
Reference compound IC50 for MEK5
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