Activity-based MEKK2 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MEKK2
Enzyme
Human MEKK2, wt
HGNC
MAP3K2
Manning ID
MAP3K2
Synonym
MEKK2, MEKK2B, MAP3K2, MAPKKK2
UniProt
Q9Y2U5 (M3K2_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Casein, ATP
Reaction
Casein + 33P-ATP = 33P-Casein + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 34 |
| Sorafenib | 10000 |
| Sunitinib | 830 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MEKK2 |
| 355 wt Profiler | Yes |
| 355 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein | Yes; ProQinaseTM – MEKK2 (Activity verified by 33PanQinaseTM-Assay) |
| Product Number | 0583-0000-1 |
| Web Shop | Reaction Biology Online Shop |
PDF