Activity-based MEKK3 kinase assay for compound screening and profiling via 33PanQinaseTM – assay technology
Target Overview
RBE Target ID
MEKK3
Enzyme
Human MEKK3, wt
HGNC
MAP3K3
Manning ID
MAP3K3
Synonym
MAPKKK3, MEKK3, MAP3K3, MEK kinase3
UniProt
Q99759 (M3K3_HUMAN)
Kinase Type
Protein kinase
Kinase Group
STE
Assay Properties
Assay Format
33PanQinaseTM
Substrates
Casein, ATP
Reaction
Casein + 33P-ATP = 33P-Casein + ADP
Readout
Enzymatic activity
Technical Reference Compound IC50 value(s)
| Compounds | IC50 (nM) |
|---|---|
| Staurosporine | 35 |
| Sorafenib | 10000 |
| Sunitinb | 810 |
Screening Location
Freiburg, Germany
Further Information
More information can be found on our website Kinase Assays
Service and Product Availability
Screening Opportunities
| FreeChoice | MEKK3 |
| 345 wt Profiler | Yes |
| 345 IC50 wt Profiler | Yes |
| Mutant Profiler Addon | No |
| Lipid Kinase Profiler Addon | No |
| IC50 Profiler | No |
Recombinant Protein Availability
| Active recombinant protein |
Yes; ProQinaseTM – MEKK3 |
| Product Number | 0859-0000-1 |
| Web Shop | Reaction Biology Online Shop |
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