MERTK NanoBRET Target Engagement Intracellular Kinase Assay for screening of compounds binding to the target.
MERTK
MERTK
MER, MER-PEN, RP38, c-Mer
HEK293 human epithelial cell line from ATCC
384-well plate format using Promega’s NanoBRET TE Intracellular Kinase Assay
HEK293 cells transiently expressing NanoLuc-MERTK Fusion Vector were seeded into the wells of 384-well plates. The cells were pre-treated with the NanoBRET Tracer K-9 and then treated with reference compound Foretinib for 1 hour. The BRET signal was measured on an Envision 2104 Multilabel Reader. IC50 value was calculated and IC50 curve was plotted using the GraphPad Prism program based on a sigmoidal dose response equation.
Binding of compound to target kinase
| Compounds | IC50 (M) |
|---|---|
| Foretinib | 1.317e-009 |
Malvern, PA, USA
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Reference compound IC50 for MERTK
